Pennsylvania Animal Diagnostic Laboratory System
PADLS User Guide Special Services Salmonella Reference Center
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7.5 The Salmonella Reference Center at NBC

The Salmonella Reference Center (SRC) located on the University of Pennsylvania’s New Bolton Center campus provides a selection of bacterial culture options for the diagnosis of Salmonella infections in animals. In addition, techniques are available for the culture of Salmonella from environmental samples for environmental monitoring and Biosecurity programs. A realtime PCR assay is available for the rapid diagnosis of Salmonella from fecal samples. The isolation, identification and characterization of Salmonella strains from animals are routinely performed using standard protocols. In addition to phenotypic typing techniques such as biochemical identification, antibiotic susceptibility testing and serotyping, modern molecular typing by Pulsed Field Gel Electrophoresis (PFGE) is provided to help in the characterization of Salmonella outbreaks. Surveillance monitoring of the development of antibiotic drug resistance is undertaken to identify emerging organisms in animals to aid in the control and prevention of disease in the Commonwealth. Support is provided to a number of State and Federal programs such as PEQAP and NPIP. In support of these SRC provides a fast track “out of hours” service. The provision of this service is controlled by an SOP and copies of the SRC unit quality manual/user guide are made available to clients. SRC collaborates in the training courses run by the NPIP.

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