Pennsylvania Animal Diagnostic Laboratory System
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9.1.1 Aquaculture - Virology

The mission of the Virology section is the provision of timely and accurate diagnoses of viral diseases. The laboratory has five working areas:

  1. avian and mammalian virology
  2. aquaculture
  3. cell culture
  4. electron microscopy
  5. biotechnology (e.g., PCR, ELISA, PAGE).

Tests and assays are arranged by a weekly schedule but emergency submissions are done upon arrival of the specimen. The cell culture laboratory maintains a variety of cell lines and SPF embryonating chicken eggs to provide virus isolate and identify viruses in a variety of species. Electron microscopy to identify viruses is used when viruses are difficult to propagate in cell cultures or to identify the morphology of new pathogens when assays are unavailable. Detection methods for viruses employing biotechnology are used to enhance sensitivity and specificity of tests and decrease turnaround of results.

1Tissue/Specimen guidelines for aquaculture virology submission are as follows:

Under 4 cmEntire fish (remove yolk sac if present)
4-6 cmEntire viscera (including kidney)
Over 6 cmKidney, spleen, and gill filaments
Sexually matureOvarian fluid, kidney, spleen, gill filaments and Milt (male reproductive fluid)

Aquaculture - Virology

Test/
Agent		SiteProcedureSpeciesTissue/
Specimen		ContainerShipping* SetupTurn-
Around		Remarks
Channel Catfish VirusPSUVI
 		Fish (catfish)As stated above1Leak-proof container with VTMOvernight on ice, do not freeze T-Th22-25 daysVTM is available through PSU and PVL.
Advance notice is preferred for this non-routine test
Infectious Hematopoietic Necrosis (IHN)PSUVI
 		Fish (Salmonid spp.)As stated above1Leak-proof container with VTM.Overnight on ice, do not freeze M-Th22-25 daysVTM is available through PSU and PVL
Infectious Pancreatic Necrosis (IPN) PSUVI
 		Fish (Salmonid spp.)As stated above1Leak-proof container with VTMOvernight on ice, do not freeze M-Th22-25 daysVTM is available through PSU and PVL
Koi Herpes Virus (KHV)PSU VI, PCRFish (carp)As stated above1Leak-proof container with VTMOvernight on ice, do not freeze M-Th22-25 daysVTM is available through PSU and PVL
Large Mouth Bass Virus (LMBV)PSU VIFish (carp)As stated above1Leak-proof container with VTMOvernight on ice, do not freeze On request22-32 daysVTM is available through PSU and PVL
Spring Viremia of Carp PSUVI, PCR
 		Fish (carp)As stated above1Leak-proof container with VTMOvernight on ice, do not freeze T-Th

 

22-25 daysVTM is available through PSU and PVL.
Advance notice is preferred for this non-routine test
Viral Hemorrhagic Septicemia (VHS)PSUVI
 		Fish (Salmonid spp.)As stated above1Leak-proof container with VTMOvernight on ice, do not freeze M-Th22-25 daysVTM is available through PSU and PVL
Viral Isolation Screen (IHN) (IPN) (VHS)PSUVIFish (Salmonid spp.)As stated above1Leak-proof container with VTMOvernight on ice, do not freeze M-Th22-25 daysVTM is available through PSU and PVL.
*If shipping to PSU, do not use the US Postal Service

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