PADLS
User Guide - Contents
Tests
Electron Microscopy
| Pennsylvania Animal Diagnostic Laboratory System | |
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PADLS
User Guide - Contents
Tests
Electron Microscopy
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Available at The Pennsylvania State University.
| Procedure | Tissue/ Specimen | Container | Shipping*** | Turn- Around | Remarks |
|---|---|---|---|---|---|
| Negative Staining | Ileum contents, feces, (10 ml) secretions, vesicular fluids, lesions or lesion scrapings, fresh tissues. (Formalin fixed tissues are not suitable for this test). | Leak-proof container | Overnight on ice, do not freeze | 2-3 days | Test is set up M-Th at PSU or by prior arrangement. |
| Thin Sectioning* | -Tissue should be fixed with fresh 2.5% glutaraldehyde in 0.1M phosphate or cacodylate buffer** -H&E stained tissue on a light microscope slide. | Leak-proof container Slide mailer | Overnight on ice, do not freeze Slide(s) and paraffin embedded tissue block sent via surface carrier, standard delivery. | 10-21 days 14 days paraffin embedded tissue | Examination of tissues is done M-Th at PSU or by prior arrangement. Buffered glutaraldehyde is available through PSU. Formalin fixed tissue can be used, however, ultrastructural morphology of tissue is poor. |
*Precut of tissue done initially to determine best sites which may contain microorganism or lesion.
**If specimen request requires immunohistochemistry analyses, tissue or specimen should be fixed in a buffered 2.0% paraformaldehyde solution. These solutions are available on request from the electron microscopy section.
***If shipping to PSU, do not use the US Postal Service
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